Craniofacial Developmental Atlas of Protein Expression of the House Finch

Expression of CaM across the house finch beak at stages HH29/30, HH32, HH36, HH39 and HH42.

HH29/30

CaM expression at HH29/30

CaM (brown stain) shows weak cytoplasmic and nuclear expression across both the upper (1) and lower jaw. (2) Blood cells show high nuclear expression. (3) No expression at the tip of the upper jaw. (4) Medium cytoplasmic and nuclear expression in myogenic condensation. (5) Weak cytoplasmic and nuclear expression in contact with low nuclear expression that is present at the mesenchyme of the outer edge in the lower jaw. Scale bars are 50µm. See expression of all proteins at stage HH29/30 here.

HH32

CaM expression at HH32

CaM (brown stain) overall (1) weak cytoplasmic and nuclear expression with some areas of (2) low nuclear expression in the upper jaw and (3, 5) no expression. (4) High nuclear expression in blood cells. Scale bars are 50µm. See expression of all proteins at stage HH32 here.

HH36

CaM expression at HH36

CaM (brown stain) shows overall weak cytoplasmic and nuclear expression, (1) including the nasal conchae but with low nuclear expression in the surrounding mesenchyme and developing nasal cartilage. (2) Medium cytoplasmic and nuclear expression in emerging osteogenic region. Weak cytoplasmic expression in developing (3) premaxillary and (6) Meckel's cartilage. No expression in (4) basihyal cartilage or tip of upper and (5) outer edge of lower jaw. Strong nuclear expression in blood cells. Scale bars are 50µm. See expression of all proteins at stage HH36 here.

HH39

CaM expression at HH39

CaM (brown stain) shows weak cytoplasmic expression in the upper jaw while weak cytoplasmic and nuclear expression in the lower jaw with no expression at the tip. Difference in expression between ectoderm and endoderm, with ectoderm lacking expression and endoderm showing weak cytoplasmic expression. (1) No expression in mesenchyme subjacent to epithelial prominences. (2) Weak cytoplasmic expression in nasal conchae and adjacent cartilage, but weak nuclear expression in nearby mesenchyme. (3) Developing premaxillary cartilage and (5) Meckel's cartilage shows weak cytoplasmic expression with no expression in adjoining mesenchyme. (4) Myogenic area and (6) epithelium with high cytoplasmic and nuclear expression. Scale bars are 50µm. See expression of all proteins at stage HH39 here.

HH42

CaM expression at HH42

CaM (brown stain) is locally expressed but shows no expression in mesenchyme of upper and lower jaw. (1) Dermal condensations, surrounding mesenchyme and epithelium show no expression. (2) Weak cytoplasmic and nuclear expression in nasal conchae. (3, 4) Moderate to strong cytoplasmic and nuclear expression in myogenic area. (5) Meckel's cartilage and mesenchyme with no expression. Scale bars are 50µm. See expression of all proteins at stage HH42 here.